People should have an idea on how to use a dissolved oxygen test kit. With the use of this material, one can easily determine the health of the aquatic ecosystem. With the result, one can help assess the pollution or productivity of areas such as lakes, streams, or rivers. To complete the testing, here are some steps one should follow.
First of all, the person should fill the BOD bottle with sample H2O. One can just dip this bottle to a stream or lake and wait for it to fill up. One should make sure that there are zero air bubbles inside the said bottle too since this will alter the results. If there are some bubbles, just tip the bottle from one side to the other to let them escape.
Prepare the manganese sulfate as well. Add this to the sample using a calibrated pipette. The said pipette should be inserted into the H2O's surface, squeeze it slowly after that. When doing this, one has to make sure that no air bubbles are being introduced in this sample to avoid altering the result.
It is also a given to pay close attention to the alkali-iodide-azide reagent. One will have to use this as this will be added to one's sample. Since the person is going to add this alkali-iodide-azide reagent into one's sample, one has to make use of the pipette as well. There should be less difficulties in adding this reagent into one's sample.
Carefully put the stopper into the bottle, making sure that there are no air introduced inside. One should then turn the BOD bottle upside down. Do this step several times just to mix its contents. Do not forget to check whether the sample has bubbles or not. If there are air bubbles, then that means one needs to discard the said sample and then start over.
Sulfuric acid will have to be added to this sample too. The person will have to utilize the calibrated pipette as well when it comes to the addition of the sulfuric acid into this sample. This time around, instead of dipping this pipette below the surface, one just needs to tip the pipette about the surface. Stopper the bottle again and turn it over, mixing the content.
Prepare an Erlenmeyer flask beforehand too. The Erlenmeyer flask is where the person will need to put a 201 ml of one's sample into. For the tester to obtain the correct measurement of the sample, the person should get a graduated cylinder and use it. Get a calibrated pipette afterward and then add the sodium thiosulfate to change the color to pale straw.
This time, one should then add starch solution into the sample. The starch solution should be in the amount of two milliliter. After adding the said solution, this sample should then turn to blue. Continue adding more sodium thiosulfate into the said solution until it becomes a clear color.
Do not forget to record how many sodium thiosulfate one has added to the solution. With this, one must be able to calculate the element content of the said sample. Since the person is going to use this dissolved oxygen test kit, assume that every sodium thiosulfate equates to one ml per liter of dissolved oxygen.
First of all, the person should fill the BOD bottle with sample H2O. One can just dip this bottle to a stream or lake and wait for it to fill up. One should make sure that there are zero air bubbles inside the said bottle too since this will alter the results. If there are some bubbles, just tip the bottle from one side to the other to let them escape.
Prepare the manganese sulfate as well. Add this to the sample using a calibrated pipette. The said pipette should be inserted into the H2O's surface, squeeze it slowly after that. When doing this, one has to make sure that no air bubbles are being introduced in this sample to avoid altering the result.
It is also a given to pay close attention to the alkali-iodide-azide reagent. One will have to use this as this will be added to one's sample. Since the person is going to add this alkali-iodide-azide reagent into one's sample, one has to make use of the pipette as well. There should be less difficulties in adding this reagent into one's sample.
Carefully put the stopper into the bottle, making sure that there are no air introduced inside. One should then turn the BOD bottle upside down. Do this step several times just to mix its contents. Do not forget to check whether the sample has bubbles or not. If there are air bubbles, then that means one needs to discard the said sample and then start over.
Sulfuric acid will have to be added to this sample too. The person will have to utilize the calibrated pipette as well when it comes to the addition of the sulfuric acid into this sample. This time around, instead of dipping this pipette below the surface, one just needs to tip the pipette about the surface. Stopper the bottle again and turn it over, mixing the content.
Prepare an Erlenmeyer flask beforehand too. The Erlenmeyer flask is where the person will need to put a 201 ml of one's sample into. For the tester to obtain the correct measurement of the sample, the person should get a graduated cylinder and use it. Get a calibrated pipette afterward and then add the sodium thiosulfate to change the color to pale straw.
This time, one should then add starch solution into the sample. The starch solution should be in the amount of two milliliter. After adding the said solution, this sample should then turn to blue. Continue adding more sodium thiosulfate into the said solution until it becomes a clear color.
Do not forget to record how many sodium thiosulfate one has added to the solution. With this, one must be able to calculate the element content of the said sample. Since the person is going to use this dissolved oxygen test kit, assume that every sodium thiosulfate equates to one ml per liter of dissolved oxygen.
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